Spinning-disk confocal microscopy is an optical imaging technique that can be used to generate detailed three-dimensional fluorescence images of living cells and their contents. Although a powerful tool for observing dynamic processes in living organisms, it has proved difficult to use for all but the thinnest biological specimens. Motivated by a need to see more deeply into living cells, Yuko Mimori-Kiyosue at the RIKEN Center for Developmental Biology and colleagues have now made major technical improvements to the technique that deliver greatly improved resolution and clarity.
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